Identification of DNA methylation markers for lineage commitment of in in vitro hepatogenesis

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dc.contributor.authorMirang Kim-
dc.contributor.authorTae-Wook Kang-
dc.contributor.authorH C Lee-
dc.contributor.authorY M Han-
dc.contributor.authorH Kim-
dc.contributor.authorH D Shin-
dc.contributor.authorH S Cheong-
dc.contributor.authorD Lee-
dc.contributor.authorSeon-Young Kim-
dc.contributor.authorYong Sung Kim-
dc.date.accessioned2017-04-19T09:23:33Z-
dc.date.available2017-04-19T09:23:33Z-
dc.date.issued2011-
dc.identifier.issn0964-6906-
dc.identifier.uri10.1093/hmg/ddr171ko
dc.identifier.urihttps://oak.kribb.re.kr/handle/201005/10171-
dc.description.abstractHepatocytes that have differentiated from human embryonic stem cells (hESCs) have great potential for the treatment of liver disease as well as for drug testing. Moreover, in vitro hepatogenesis is a powerful model system for studying the molecular mechanisms underlying liver development. DNA methylation is an important epigenetic mechanism that influences differential gene expression during embryonic development. We profiled gene expression and DNA methylation of three cell states of in vitro hepatogenesis-hESC, definitive endoderm and hepatocyte-using microarray analysis. Among 525 state-specific expressed genes, 67 showed significant negative correlation between gene expression and DNA methylation. State-specific expression and methylation of target genes were validated by quantitative reverse transcription-polymerase chain reaction and pyrose- quencing, respectively. To elucidate genome-scale methylation changes beyond the promoter, we also per- formed high-throughput sequencing of methylated DNA captured by the MBD2 protein. We found dynamic methylation changes in intergenic regions of the human genome during differentiation. This study provides valuable methylation markers for the lineage commitment of in vitro hepatogenesis and should help elucidate the molecular mechanisms underlying stem cell differentiation and liver development.-
dc.publisherOxford Univ Press-
dc.titleIdentification of DNA methylation markers for lineage commitment of in in vitro hepatogenesis-
dc.title.alternativeIdentification of DNA methylation markers for lineage commitment of in in vitro hepatogenesis-
dc.typeArticle-
dc.citation.titleHuman Molecular Genetics-
dc.citation.number14-
dc.citation.endPage2733-
dc.citation.startPage2722-
dc.citation.volume20-
dc.contributor.affiliatedAuthorMirang Kim-
dc.contributor.affiliatedAuthorTae-Wook Kang-
dc.contributor.affiliatedAuthorSeon-Young Kim-
dc.contributor.affiliatedAuthorYong Sung Kim-
dc.contributor.alternativeName김미랑-
dc.contributor.alternativeName강태욱-
dc.contributor.alternativeName이한철-
dc.contributor.alternativeName한용만-
dc.contributor.alternativeName김혜민-
dc.contributor.alternativeName신형두-
dc.contributor.alternativeName정현섭-
dc.contributor.alternativeName이대엽-
dc.contributor.alternativeName김선영-
dc.contributor.alternativeName김용성-
dc.identifier.bibliographicCitationHuman Molecular Genetics, vol. 20, no. 14, pp. 2722-2733-
dc.identifier.doi10.1093/hmg/ddr171-
dc.description.journalClassY-
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Aging Convergence Research Center > 1. Journal Articles
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