Identification of DNA methylation markers for lineage commitment of in in vitro hepatogenesis

Cited 11 time in scopus
Metadata Downloads
Title
Identification of DNA methylation markers for lineage commitment of in in vitro hepatogenesis
Author(s)
Mirang Kim; Tae-Wook Kang; H C Lee; Y M Han; H Kim; H D Shin; H S Cheong; D Lee; Seon-Young Kim; Yong Sung Kim
Bibliographic Citation
Human Molecular Genetics, vol. 20, no. 14, pp. 2722-2733
Publication Year
2011
Abstract
Hepatocytes that have differentiated from human embryonic stem cells (hESCs) have great potential for the treatment of liver disease as well as for drug testing. Moreover, in vitro hepatogenesis is a powerful model system for studying the molecular mechanisms underlying liver development. DNA methylation is an important epigenetic mechanism that influences differential gene expression during embryonic development. We profiled gene expression and DNA methylation of three cell states of in vitro hepatogenesis-hESC, definitive endoderm and hepatocyte-using microarray analysis. Among 525 state-specific expressed genes, 67 showed significant negative correlation between gene expression and DNA methylation. State-specific expression and methylation of target genes were validated by quantitative reverse transcription-polymerase chain reaction and pyrose- quencing, respectively. To elucidate genome-scale methylation changes beyond the promoter, we also per- formed high-throughput sequencing of methylated DNA captured by the MBD2 protein. We found dynamic methylation changes in intergenic regions of the human genome during differentiation. This study provides valuable methylation markers for the lineage commitment of in vitro hepatogenesis and should help elucidate the molecular mechanisms underlying stem cell differentiation and liver development.
ISSN
0964-6906
Publisher
Oxford Univ Press
DOI
http://dx.doi.org/10.1093/hmg/ddr171
Type
Article
Appears in Collections:
Aging Convergence Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.