Silver-stained fibrin zymography : separation of proteases and activity detection using a single substrate-containing gel = Silver-stained fibrin zymography : 단일기질 함유 겔을 이용한 프로테아제의 활성 검증 및 분리

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Title
Silver-stained fibrin zymography : separation of proteases and activity detection using a single substrate-containing gel = Silver-stained fibrin zymography : 단일기질 함유 겔을 이용한 프로테아제의 활성 검증 및 분리
Author(s)
Dong Min Chung; K E Kim; Keug Hyun Ahn; Chan Sun Park; D H Kim; H B Koh; Hyo Kon Chun; Byung Dae Yoon; H J Kim; Min-Soo Kim; N S Choi
Bibliographic Citation
Biotechnology Letters, vol. 33, no. 8, pp. 1663-1666
Publication Year
2011
Abstract
A new zymogram method, silver-stained fibrin zymography, for separation of protease bands and activity detection using a single substrate gel, was developed. The method takes advantage of the nanoscale sensitivity of both zymography and silver staining. After SDS-PAGE in a gel containing fibrin, the gel was incubated in enzyme reaction buffer and the zymogram was silver-stained. Bands with protease activity were stained with silver in clear areas where the protein substrate had been degraded. The molecular sizes of proteases were accurately determined. Furthermore, proteases of high molecular weight were clearly and sharply resolved.
Keyword
ProteaseSilver stainingZymography
ISSN
0141-5492
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10529-011-0619-3
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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