Photobacterium atrarenae sp. nov. a novel bacterium isolated from sea sand = Sea sand에서 분리된 신종 세균 Photobacterium atrarenae

Abstract

The gram-reaction-negative, motile, facultatively anaerobic, catalase-positive, oxidase-positive bacterial strain M3-4 T was isolated from black sea sand and subjected to a taxonomic study. Cells of strain M3-4 T have monotrichous flagella, grow optimally at 37°C and at pH 7-8 in the presence of 1-4% (w/v) NaCl and hydrolyze casein, starch and L-tyrosine. According to phylogenetic analyses using 16S rRNA gene sequences, strain M3-4 T belongs to the genus Photobacterium and is most closely related to Photobacterium rosenbergii LMG 22223 T (97.4%) and P. gaetbulicola KCTC 22804 T (96.6%). The DNA-DNA relatedness value between M3-4 T and P. rosenbergii LMG 22223 T was 21.5%. The DNA G+C mol% of strain M3-4 T was 53.6. The major cellular fatty acid of strain M3-4 T was a summed feature 3 consisting of C 16:1 ω7c and/or iso-C 15:0 2-OH (35.0%), followed by C 16:0 (25.4%) and C 18:1ω7c (16.8%). These data suggest that strain M3-4 T represents a novel species in genus Photobacterium, for which the name P. atrarenae sp. nov. is proposed. The type strain is M3-4 T (= KCTC 23265 T = NCAIM B 02414 T).