Spectrophotometric assay for determination of chlorogenic acid using green pigment formation and quantitative analysis of chlorogenic acid in blueberry leaf = 녹색반응 이용한 클로로겐산 함량측정을 위한 흡광도 분석법과 블루베리 잎의 클로로겐산 함량분석

Abstract

We developed a spectrophotometric assay for the quantitative determination of chlorogenic acid based on the formation of green pigment at 50oC under glycine and alkaline conditions in 96-well plates. The formation of green pigment was linear with a series of chlorogenic acid concentration (0-300 μM). Using this method, the content of chlorogenic acid (12.42 mg/g dry weight) in the leaves of blueberry was quantified. This method is high-throughput, cost-effective, rapid, and easy to perform