Spectrophotometric assay for determination of chlorogenic acid using green pigment formation and quantitative analysis of chlorogenic acid in blueberry leaf = 녹색반응 이용한 클로로겐산 함량측정을 위한 흡광도 분석법과 블루베리 잎의 클로로겐산 함량분석

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Title
Spectrophotometric assay for determination of chlorogenic acid using green pigment formation and quantitative analysis of chlorogenic acid in blueberry leaf = 녹색반응 이용한 클로로겐산 함량측정을 위한 흡광도 분석법과 블루베리 잎의 클로로겐산 함량분석
Author(s)
Dong Min Chung; Y C Chung; Hyo Kon Chun
Bibliographic Citation
Korean Journal of Life Sciences, vol. 21, no. 4, pp. 610-612
Publication Year
2011
Abstract
We developed a spectrophotometric assay for the quantitative determination of chlorogenic acid based on the formation of green pigment at 50oC under glycine and alkaline conditions in 96-well plates. The formation of green pigment was linear with a series of chlorogenic acid concentration (0-300 μM). Using this method, the content of chlorogenic acid (12.42 mg/g dry weight) in the leaves of blueberry was quantified. This method is high-throughput, cost-effective, rapid, and easy to perform
Keyword
Blueberry leafchlorogenic acidgreen pigmentamino acid96 well plate
ISSN
I000-0052
Publisher
South Korea
DOI
http://dx.doi.org/10.5352/JLS.2011.21.4.610
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
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