Major chimpanzee-specific structural changes in sperm development-associated genes

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Title
Major chimpanzee-specific structural changes in sperm development-associated genes
Author(s)
Ryong Nam Kim; D W Kim; Sang-Haeng Choi; S H Chae; Seong-Hyeuk Nam; Dong-Wook Kim; Ae-Ri Kim; Aram Kang; Kun Hyang Park; Y S Lee; M Hirai; Y Suzuki; S Sugano; K Hashimoto; Dae Soo Kim; Hong-Seog Park
Bibliographic Citation
Functional & Integrative Genomics, vol. 11, no. 3, pp. 507-517
Publication Year
2011
Abstract
A comprehensive analysis of transcriptional structures of chimpanzee sperm development-associated genes is of significant interest for deeply understanding sperm development and male reproductive process. In this study, we sequenced 7,680 clones from a chimpanzee testis full-length cDNA library and obtained 1,933 nonredundant high-quality full-length cDNA sequences. Comparative analysis between human and chimpanzee showed that 78 sperm developmentassociated genes, most of which were yet uncharacterized, had undergone severe structural changes (mutations at the start/stop codons, INDELs, alternative splicing variations and fusion forms) on genomic and transcript levels throughout chimpanzee evolution. Specifically, among the 78 sperm development-associated genes, 39 including ODF2, UBC, and CD59 showed markedly chimpanzee-specific structural changes. Through dN/dS analysis, we found that 56 transcripts (including seven sperm development-associated genes) had values of greater than one when comparing human and chimpanzee DNA sequences, whereas the values were less than one when comparing humans and orangutans. Gene ontology annotation and expression profiling showed that the chimpanzee testis transcriptome was enriched with genes that are associated with chimpanzee male germ cell development. Taken together, our study provides the first comprehensive molecular evidence that many chimpanzee sperm development-associated genes had experienced severe structural changes over the course of evolution on genomic and transcript levels.
Keyword
ChimpanzeeGene ontologySperm developmentStructural changesTestis
ISSN
1438-793X
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s10142-011-0220-9
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > Core Research Facility & Analysis Center > 1. Journal Articles
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