Production of recombinant human keratinocyte growth factor from Bombyx mori (Lepidopera: Bombycidae) Bm5 cells = 누에 배양세포에서 인간형 재조합단백질 각질세포 성장인자 생산

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Title
Production of recombinant human keratinocyte growth factor from Bombyx mori (Lepidopera: Bombycidae) Bm5 cells = 누에 배양세포에서 인간형 재조합단백질 각질세포 성장인자 생산
Author(s)
S Y Han; C Y Jin; K Kwon; E Y Yun; T W Goo; S W Kim; J S Choi; Kweon Yu; O Y Kwon
Bibliographic Citation
Journal of Life Science, vol. 21, no. 6, pp. 907-911
Publication Year
2011
Abstract
Using silkworm Bombyx mori Bm5 cells, we established a stable cell line expressing the human keratinocyte growth factor (hKGF), named by the Bm5-hKGF cell, in which the protein hKGF is synthesized in the cell and secreted in the cell culture supernatant (CCS) at approximately 15-20 ng/ml. When the Bm5-hKGF cell was co-expressed with B. mori protein disulfide isomerase (bPDI) cDNA, its secretion increased by about two times the original amount. Through wound healing migration assay, it was demonstrated that the secreted hKGF included in the CCS has a very powerful biological activity of keratinocyte proliferation. We expect to produce useful human recombinant proteins from silkworm cultured cells in large quantities at low prices.
Keyword
Silkworm Bombyx mori Bm5 cellshuman keratinocyte growth factorprotein disulfide isomerase
ISSN
I000-0128
Publisher
South Korea
DOI
http://dx.doi.org/10.5352/JLS.2011.21.6.907
Type
Article
Appears in Collections:
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
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