Rapid concentration of some bacteriocin-like compounds using an organic solvent

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Title
Rapid concentration of some bacteriocin-like compounds using an organic solvent
Author(s)
Dong Min Chung; K E Kim; Seong Yoep Jeong; C S Park; Keug Hyun Ahn; D H Kim; D O Kang; Hyo Kon Chun; Byung Dae Yoon; H B Koh; H J Kim; N S Choi
Bibliographic Citation
Food Science and Biotechnology, vol. 20, no. 5, pp. 1457-1459
Publication Year
2011
Abstract
A new solvent extraction method for isolation of bacteriocin-like compounds (BLCs) from bacterial culture broth was developed. Culture supernatant of Pediococcus acidilactici WRL-1 was mixed with 3 volumes of cold acetone and maintained at ?20°C for 2 h. The solvent and precipitate were separated by centrifugation. Most (95.2 ±2.5%) pediocin activity was found in the liquid phase and it was obtained by evaporation. This method was also an effective means of isolating other BLCs from cultures of Lactobacillus acidophilus, Lactobacillus plantarum, and Bacillus licheniformis DJ-2. Other solvents (methanol, ethanol, and butanol) were also suitable toward this end.
Keyword
anti-microbialBacillus cereusbacteriocin-like compoundorganic solvent
ISSN
1226-7708
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.1007/s10068-011-0201-8
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
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