The use of cotyledonary-node explants in Agrobacterium tumefaciens-mediated transformation of cucumber (Cucumis sativus L.) = Agrobacterium에 의한 오이 형질전환에서 자엽절 절편의 이용

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Title
The use of cotyledonary-node explants in Agrobacterium tumefaciens-mediated transformation of cucumber (Cucumis sativus L.) = Agrobacterium에 의한 오이 형질전환에서 자엽절 절편의 이용
Author(s)
H A Jang; Hyun A Kim; Suk Yoon Kwon; D W Choi; P S Choi
Bibliographic Citation
Journal of Plant Biotechnology, vol. 38, no. 3, pp. 198-202
Publication Year
2011
Abstract
Agrobacterium tumefaciens-mediated cotyledonary-node explants transformation was used to produce transgenic cucumber. Cotyledonary-node explants of cucumber (Cucumis sativus L. cv., Eunsung) were co-cultivated with Agrobacterium strains (EHA101) containing the binary vector (pPZP211) carrying with CaMV 35S promoter-nptII gene as selectable marker gene and 35S promoter-DQ gene (unpublished data) as target gene. The average of transformation efficiency (4.01%) was obtained from three times experiments and the maximum efficiency was shown at 5.97%. A total of 9 putative transgenic plants resistant to paromomycin were produced from the cultures of cotyledonary-node explants on selection medium. Among them, 6 transgenic plants showed that the nptII gene integrated into each genome of cucumber by Southern blot analysis.
Keyword
Agrobacteriumcotyledonary-nodeparomomycintransgenic cucumber
ISSN
1229-2818
Publisher
South Korea
DOI
http://dx.doi.org/10.5010/JPB.2011.38.3.198
Type
Article
Appears in Collections:
Division of Biomaterials Research > 1. Journal Articles
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