Preparative isolation of 8-methoxypsoralen from the rhizomes of Pulsatilla chinensis using high-speed counter-current chromatography

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Title
Preparative isolation of 8-methoxypsoralen from the rhizomes of Pulsatilla chinensis using high-speed counter-current chromatography
Author(s)
G H Quan; Sei Ryang Oh; Hyuk Hwan Song; Hyeong Kyu Lee; Y W Chin
Bibliographic Citation
Journal of Korean Society for Applied Biological Chemistry, vol. 54, no. 4, pp. 623-627
Publication Year
2011
Abstract
A simple and rapid purification method of 8-methoxy-psoralen from the crude methanol extract of the rhizomes of Pulsatilla chinenis was established using high-speed counter-current chromatography (HSCCC). From the crude extract (233.2 mg), 8-methoxypsoralen (10.0 mg) was separated for the first time in one-step, using a two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water (5:5:5:5, v/v/v/v). The isolated amount of this compound was found to be equivalent to 89% of the amount observed quantitatively in the crude methanolic extract. The final purity of the 8-methoxy-psoralen was determined to be over 98% by ultra-performance liquid chromatography-UV analysis.
Keyword
8-methoxypsoralenHigh-speed counter-current chromatographyPulsatilla chinensis
ISSN
I000-0171
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.3839/jksabc.2011.094
Type
Article
Appears in Collections:
Ochang Branch Institute > 1. Journal Articles
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