Optimization of culture conditions for 1,3-propanediol production from glycerol using a mutant strain of Klebsiella pneumoniae

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Title
Optimization of culture conditions for 1,3-propanediol production from glycerol using a mutant strain of Klebsiella pneumoniae
Author(s)
Baek Rock OhJeong-Woo SeoSun-Yeon Heo; Won Kyung Hong; L H Luo; Seonghun Kim; D H Park; Chul Ho Kim
Bibliographic Citation
Applied Biochemistry and Biotechnology, vol. 166, no. 1, pp. 127-137
Publication Year
2012
Abstract
In the present work, mutant strains of Klebsiella pneumoniae with deletions of the als gene encoding acetolactate synthase involved in synthesis of 2,3-butanediol, the ldhA gene encoding lactate dehydrogenase required for lactate synthesis, or both genes, were prepared. Production of 1,3-propanediol (1,3-PD) from glycerol was enhanced in the ldhA mutant strain (ΔldhA), but lower in Δals or Δals ΔldhA mutant strains compared to the parent strain, concomitant with a reduction in the glycerol consumption rate, indicating that deletion of ldhA alone was useful to improve 1,3-PD production. Fed-batch fermentation analysis revealed that, in the ΔldhA mutant strain, 1,3-PD production was higher at low pH than at neutral pH; the reverse was true for the parent strain. Further optimization of culture conditions, by variation of aeration and glycerol feed rates, dramatically improved the production of 1,3-PD by the mutant strain. The maximum level attained was 102.7 g l-1 of 1,3-PD from glycerol.
Keyword
1,3-PropanediolGene deletionGlycerolKlebsiella pneumoniaeOptimized culture conditions
ISSN
0273-2289
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s12010-011-9409-6
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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