Norkurarinol inhibits toll-like receptor 3 (TLR3)-mediated pro-inflammatory signaling pathway and rotavirus replication

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Title
Norkurarinol inhibits toll-like receptor 3 (TLR3)-mediated pro-inflammatory signaling pathway and rotavirus replication
Author(s)
Hyun Mee Oh; Seung Woong Lee; M H Park; Mi-Hwa Kim; Young Bae Ryu; M S Kim; H H Kim; K H Park; Woo Song LeeSu-Jin ParkMun Chual Rho
Bibliographic Citation
Journal of Pharmacological Sciences, vol. 118, no. 2, pp. 161-170
Publication Year
2012
Abstract
This study examined the effect of norkurarinol on the toll-like receptor 3 (TLR3)- mediated signaling pathways and rotavirus replication. Norkurarinol, a lavandulylated flavanone, was isolated from the roots of Sophora flavescens, which has been shown to have anti-inflammatory activity. Norkurarinol suppressed the NF-κB and AP-1 inducible secreted embryonic alkaline phosphatase (SEAP) activity induced by poly(I:C), TLR3 ligand, in THP1-Blue-CD14 cells with IC 50 values of 20.9 μM. Norkurarinol also significantly suppressed the mRNA expression of proinflammatory and adhesive molecules induced by poly(I:C) and rotavirus infection. Pretreatment of norkurarinol blocked the NF-κB and AP-1 signaling pathway and the phosphorylation of MAPKs induced by poly(I:C). On the other hand, norkurarinol increased the level of IRF3 phosphorylation and IFNβ expression in a dose-dependent manner. Moreover, norkurarinol inhibited the rotavirus-induced cytopathic effects. These results suggest that norkurarinol can modulate the TLR3-mediated inflammatory responses and rotavirus replication.
Keyword
dsRNAInflammatory responseNorkurarinolRotavirusToll-like receptor 3 (TLR3)
ISSN
1347-8613
Publisher
Japanese Pharmacological Soc
DOI
http://dx.doi.org/10.1254/jphs.11077FP
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Immunoregulatory materials Research Center > 1. Journal Articles
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
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