Anti-allergic effect of lambertianic acid from Thuja orientalis in mouse bone marrow-derived mast cells

Abstract

Lambertianic acid is a bioactive diterpene found in the leaves of Thuja orientalis. Its effect on the bone marrow-derived mast cell (BMMC) mediated allergy and inflammation mechanism remains unknown. In this study, lambertianic acid was evaluated for its effect on the allergicmediators, including prostaglandin D2 (PGD2), leukotriene C4 (LTC4), β-hexosaminidase (β-Hex) and cyclooxygenase-2 (COX-2) protein, in phorbol 12-myristate 13-acetate (PMA) plus calcimycin-stimulated BMMCs. The results revealed that lambertianic acidinhibited the production of interleukin-6 (IL-6), PGD2 and LTC4, the expression of COX-2 and the degranulation of β-hexosaminidase in the PMA plus calcimycin-induced BMMCs. Taken together, these findings implied that lambertianic acid may possess the potential in the treatment of allergy.