Large increase in Leuconostoc citreum KM20 dextransucrase activity achieved by changing the strain/inducer combination in an E. coli expression system = E. coli strain과 유도제의 조합에 의한 글루칸 합성효소의 대량발현

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Title
Large increase in Leuconostoc citreum KM20 dextransucrase activity achieved by changing the strain/inducer combination in an E. coli expression system = E. coli strain과 유도제의 조합에 의한 글루칸 합성효소의 대량발현
Author(s)
Jin A Ko; Hyung Jae JungYoung Bae RyuSu-Jin Park; Y J Wee; D Kim; Young Min Kim; Woo Song Lee
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 22, no. 4, pp. 510-515
Publication Year
2012
Abstract
A recombinant putative dextransucrase (DexT) was produced from Leuconostoc citreum KM20 as a 160 kDa protein, but its productivity was very low (264 U/l). For optimization, we examined enzyme activity in 7 Escherichia coli strains with inducer molecules such as lactose or IPTG. E. coli BL21-CodonPlus(DE3)-RIL exhibited the highest enzyme activity with lactose. Finally, DexT activity was remarkably increased by 12-fold under the optimized culture conditions of a cell density to start induction (OD 600) of 0.95, a lactose concentration of 7.5 mM, and an induction temperature of 17°C. These results may effectively apply to the heterologous expression of other large DexT genes.
Keyword
DextransucraseLactoseLeuconostoc citreum KM20OptimizationResponse surface methodology
ISSN
1017-7825
Publisher
South Korea
DOI
http://dx.doi.org/10.4014/jmb.1111.11032
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
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