Comparative proteomic analysis of human somatic cells, induced pluripotent stem cells, and embryonic stem cells

Cited 20 time in scopus
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Title
Comparative proteomic analysis of human somatic cells, induced pluripotent stem cells, and embryonic stem cells
Author(s)
Sun Young Kim; Min-Jeong Kim; Hyeyun Jung; Won Kon Kim; S O Kwon; Myung Jin Son; I S Jang; J S Choi; Sung Goo ParkByoung Chul Park; Y M Han; Sang Chul LeeYee Sook ChoKwang-Hee Bae
Bibliographic Citation
Stem Cells and Development, vol. 21, no. 8, pp. 1272-1286
Publication Year
2012
Abstract
Induced pluripotent stem cells (iPSCs) are somatic cells that have been reprogrammed to a pluripotent state via introduction of defined transcription factors. iPSCs are a valuable resource for regenerative medicine, but whether iPSCs are identical to embryonic stem cells (ESCs) remains unclear. In this study, we performed comparative proteomic analyses of human somatic cells [human newborn foreskin fibroblasts (hFFs)], human iPSCs (hiPSCs) derived from hFFs, and H9 human ESCs (hESCs). We reprogrammed hFFs to a pluripotent state using 4 core transcription factors: Oct4 (O), Sox2 (S), Klf4 (K), and c-Myc (M). The proteome of hiPSCs induced by 4 core transcription factors was relatively similar to that of hESCs. However, several proteins, including dUTPase, GAPDH, and FUSE binding protein 3, were differentially expressed between hESCs and hiPSCs, implying that hiPSCs are not identical to hESCs at the proteomic level. The proteomes of iPSCs induced by introducing 3, 5, or 6 transcription factors were also analyzed. Our proteomic profiles provide valuable insight into the factors that contribute to the similarities and differences between hESCs and hiPSCs and the mechanisms of reprogramming.
ISSN
1525-8165
Publisher
Mary Ann Liebert, Inc
DOI
http://dx.doi.org/10.1089/scd.2011.0243
Type
Article
Appears in Collections:
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
Division of Research on National Challenges > Stem Cell Convergenece Research Center > 1. Journal Articles
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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