Active inclusion body formation using Paenibacillus polymyxa PoxB as a fusion partner in Escherichia coli

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Title
Active inclusion body formation using Paenibacillus polymyxa PoxB as a fusion partner in Escherichia coli
Author(s)
Soo-Young Park; Seung Hwan Park; Soo Keun Choi
Bibliographic Citation
Analytical Biochemistry, vol. 426, no. 1, pp. 63-65
Publication Year
2012
Abstract
Overexpression of Paenibacillus polymyxa PoxB in Escherichia coli induced the formation of inclusion bodies. An enzyme assay showed that the inclusion bodies exhibited PoxB activity, indicating that they were biologically active. Fusion of GFP and Bacillus subtilis AmyE to the C-terminus of the PoxB also induced the formation of biologically active aggregates when they were overexpressed in E. coli. Therefore, P. polymyxa PoxB can be used as a fusion partner to promote the formation of active inclusion bodies in E. coli.
Keyword
Active inclusion bodyPaenibacillus polymyxaPoxBRecombinant protein expression
ISSN
0003-2697
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.ab.2012.04.002
Type
Article
Appears in Collections:
Division of Research on National Challenges > Infectious Disease Research Center > 1. Journal Articles
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