Enzymatic synthesis of puerarin glucosides using Leuconostoc dextransucrase = 덱스트란수크라제를 활용한 퓨라닌 글루코사이드의 효소적 합성

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Title
Enzymatic synthesis of puerarin glucosides using Leuconostoc dextransucrase = 덱스트란수크라제를 활용한 퓨라닌 글루코사이드의 효소적 합성
Author(s)
Jin-A Ko; Young Bae Ryu; Tae Soon Park; Hyung Jae Jung; Jang Hoon Kim; Su-Jin ParkJoong Su Kim; D Kim; Young Min Kim; Woo Song Lee
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 22, no. 9, pp. 1224-1229
Publication Year
2012
Abstract
Puerarin (P), an isoflavone derived from kudzu roots, has strong biological activities, but its bioavailability is often limited by its low water solubility. To increase its solubility, P was glucosylated by three dextransucrases from Leuconostoc or Streptococcus species. Leuconostoc lactis EG001 dextransucrase exhibited the highest productivity of puerarin glucosides (P-Gs) among the three tested enzymes, and it primarily produced two P-Gs with a 53% yield. Their structures were identified as α-D-glucosyl- (1→6)-P (P-G) by using LC-MS or 1H- or 13C-NMR spectroscopies and α-D-isomaltosyl-(1→6)-P (P-IG2) by using specific enzymatic hydrolysis, and their solubilities were 15- and 202-fold higher than that of P, respectively. P-G and P-IG2 are easily applicable in the food and pharmaceutical industries as alternative functional materials.
Keyword
DextransucraseLeuconostoc lactisPuerarinTransglucosylationWater solubility
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.4014/jmb.1202.02007
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
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