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- Enzymatic synthesis of puerarin glucosides using Leuconostoc dextransucrase = 덱스트란수크라제를 활용한 퓨라닌 글루코사이드의 효소적 합성
- Jin-A Ko; Young Bae Ryu; Tae Soon Park; Hyung Jae Jung; Jang Hoon Kim; Su-Jin Park; Joong Su Kim; D Kim; Young Min Kim; Woo Song Lee
- Bibliographic Citation
- Journal of Microbiology and Biotechnology, vol. 22, no. 9, pp. 1224-1229
- Publication Year
- Puerarin (P), an isoflavone derived from kudzu roots, has strong biological activities, but its bioavailability is often limited by its low water solubility. To increase its solubility, P was glucosylated by three dextransucrases from Leuconostoc or Streptococcus species. Leuconostoc lactis EG001 dextransucrase exhibited the highest productivity of puerarin glucosides (P-Gs) among the three tested enzymes, and it primarily produced two P-Gs with a 53% yield. Their structures were identified as α-D-glucosyl- (1→6)-P (P-G) by using LC-MS or 1H- or 13C-NMR spectroscopies and α-D-isomaltosyl-(1→6)-P (P-IG2) by using specific enzymatic hydrolysis, and their solubilities were 15- and 202-fold higher than that of P, respectively. P-G and P-IG2 are easily applicable in the food and pharmaceutical industries as alternative functional materials.
- DextransucraseLeuconostoc lactisPuerarinTransglucosylationWater solubility
- Korea Soc-Assoc-Inst
- Appears in Collections:
- Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
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