Inducible biosynthetic nanoscaffolds as recruitment platforms for detecting molecular target interactions inside living cells = 세포내 상호작용 감지를 위한 나노 플랫폼

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Title
Inducible biosynthetic nanoscaffolds as recruitment platforms for detecting molecular target interactions inside living cells = 세포내 상호작용 감지를 위한 나노 플랫폼
Author(s)
S Lee; J S Ha; Seung Goo Lee; T K Kim
Bibliographic Citation
Journal of American Chemical Society, vol. 134, no. 28, pp. 11346-11349
Publication Year
2012
Abstract
We present a novel phenotypic readout using inducible, biosynthetic nanoscaffolds to directly visualize dynamic molecular interactions within living cells at the single-cell level with high sensitivity and selectivity. Labeled ferritin is used to form biological nanoparticles inside cells. Specific supramolecular assembly of ferritin-derived nanoparticles induces highly clustered nanoscaffolds. These inducible biosynthetic nanoscaffolds are used as the artificial recruitment/redistribution platform for monitoring interactions of a small molecule with its target protein(s) inside living cells.
ISSN
0002-7863
Publisher
Amer Chem Soc
DOI
http://dx.doi.org/10.1021/ja303518d
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > 1. Journal Articles
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