Inheritance of mitochondrial DNA in serially recloned pigs by somatic cell nuclear transfer (SCNT)

Cited 8 time in scopus
Metadata Downloads
Title
Inheritance of mitochondrial DNA in serially recloned pigs by somatic cell nuclear transfer (SCNT)
Author(s)
Minhwa Do; Won-Gu Jang; J H Hwang; Hoon Jang; Eun Jung Kim; Eun Jeong Jeong; H Shim; S S Hwang; K B Oh; S J Byun; J H Kim; Jeong Woong Lee
Bibliographic Citation
Biochemical and Biophysical Research Communications, vol. 424, no. 3, pp. 765-770
Publication Year
2012
Abstract
Somatic cell nuclear transfer (SCNT) has been established for the transmission of specific nuclear DNA. However, the fate of donor mitochondrial DNA (mtDNA) remains unclear. Here, we examined the fate of donor mtDNA in recloned pigs through third generations. Fibroblasts of recloned pigs were obtained from offspring of each generation produced by fusion of cultured fibroblasts from a Minnesota miniature pig (MMP) into enucleated oocytes of a Landrace pig. The D-loop regions from the mtDNA of donor and recipient differ at nucleotide sequence positions 16050 (A→T), 16062 (T→C), and 16135 (G→A). In order to determine the fate of donor mtDNA in recloned pigs, we analyzed the D-loop region of the donor's mtDNA by allele-specific PCR (AS-PCR) and real-time PCR. Donor mtDNA was successfully detected in all recloned offspring (F1, F2, and F3). These results indicate that heteroplasmy that originate from donor and recipient mtDNA is maintained in recloned pigs, resulting from SCNT, unlike natural reproduction.
Keyword
Mitochondrial DNARecloned pigSomatic cell nuclear transfer
ISSN
0006-291X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bbrc.2012.07.031
Type
Article
Appears in Collections:
Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.