Bacteroides thetaiotaomicron VPI-5482 glycoside hydrolase family 66 homolog catalyzes dextranolytic and cyclization reactions = 덱스트란나아제 및 환상올리고당 합성반응을 촉매하는 Bacteroides thetaiotaomicron VPI-5482 유래 효소

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Title
Bacteroides thetaiotaomicron VPI-5482 glycoside hydrolase family 66 homolog catalyzes dextranolytic and cyclization reactions = 덱스트란나아제 및 환상올리고당 합성반응을 촉매하는 Bacteroides thetaiotaomicron VPI-5482 유래 효소
Author(s)
Young Min Kim; E Yamamoto; M S Kang; H Nakai; W Saburi; M Okuyama; H Mori; K Funane; M Momma; Z Fujimoto; M Kobayashi; D Kim; A Kimura
Bibliographic Citation
FEBS Journal, vol. 279, pp. 3185-3191
Publication Year
2012
Abstract
Bacteroides thetaiotaomicron VPI-5482 harbors a gene encoding a putative cycloisomaltooligosaccharide glucanotransferase (BT3087) belonging to glycoside hydrolase family 66. The goal of the present study was to characterize the catalytic properties of this enzyme. Therefore, we expressed BT3087 (recombinant endo-dextranase from Bacteroides thetaiotaomicron VPI-5482) in Escherichia coli and determined that recombinant endo-dextranase from Bacteroides thetaiotaomicron VPI-5482 preferentially synthesized isomaltotetraose and isomaltooligosaccharides (degree of polymerization > 4) from dextran. The enzyme also generated large cyclic isomaltooligosaccharides early in the reaction. We conclude that members of the glycoside hydrolase 66 family may be classified into three types: (a) endo-dextranases, (b) dextranases possessing weak cycloisomaltooligosaccharide glucanotransferase activity, and (c) cycloisomaltooligosaccharide glucanotransferases.
Keyword
Bacteroides thetaiotaomicron VPI-5482cycloisomaltooligosaccharide glucanotransferaseendo-dextranaseglycoside hydrolase family 66isomaltotetraose
ISSN
1742-464X
Publisher
Wiley
DOI
http://dx.doi.org/10.1111/j.1742-4658.2012.08698.x
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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