Humanization by guided selections

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Title
Humanization by guided selections
Author(s)
Sang Jick Kim; H J Hong
Bibliographic Citation
Methods in Molecular Biology, vol. 907, pp. 247-257
Publication Year
2012
Abstract
Guided selection provides a powerful tool for humanization of the preexisting nonhuman antibodies as exemplified by HUMIRA, the world's first human antibody approved. This chapter describes the sequential guided selection procedure in which mouse VL and VH domains are replaced sequentially with human VL and VH, respectively to derive completely human antibody. The detailed protocols for construction of phage-displayed antibody library, panning, screening, and characterization, are included to achieve successful selection of human antibody with similar characteristics to original mouse antibody.
Keyword
Antibody libraryChain shufflingGuided selectionHuman antibodyPanningPhage display
ISSN
1064-3745
Publisher
Humana Press
DOI
http://dx.doi.org/10.1007/978-1-61779-974-7-14
Type
Article
Appears in Collections:
Division of Biomaterials Research > Synthetic Biology and Bioengineering Research Center > 1. Journal Articles
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