Direct-write patterning of bacterial cells by dip-pen nanolithography

Cited 33 time in scopus
Metadata Downloads
Title
Direct-write patterning of bacterial cells by dip-pen nanolithography
Author(s)
J Kim; Y H Shin; S H Yun; D S Choi; J H Nam; S R Kim; S K Moon; Bong Hyun Chung; J H Lee; J H Kim; K Y Kim; K M Kim; J H Lim
Bibliographic Citation
Journal of American Chemical Society, vol. 134, no. 40, pp. 16500-16503
Publication Year
2012
Abstract
The ability of dip-pen nanolithography (DPN) to generate nano- or microarrays of soft or hard materials (e.g., small molecules, DNA, proteins, nanoparticles, sols, and polymers) in a direct-write manner has been widely demonstrated. The transporting of large-sized ink materials such as bacteria, however, remains a significant challenge with this technique. The size limitation of the water meniscus formed between the DPN tip and the solid surface becomes a bottleneck in such diffusion-based molecular transport experiments. Herein, we report a straightforward "stamp-on" DPN method that uses a nanostructured poly(2-methyl-2-oxazoline) hydrogel-coated tip and carrier agents to generate patterns of micrometer-sized Escherichia coli JM 109 bacterial cells. We demonstrate that this approach enables the deposition of a single bacterial cell array on a solid surface or arrays of layers of multiple cells by modulating the viscosity of the "ink" solution. Fluorescence microscopy images indicated that the deposited bacterial cells were kept alive on Luria-Bertani-agar layered solid surfaces after DPN patterning.
ISSN
0002-7863
Publisher
Amer Chem Soc
DOI
http://dx.doi.org/10.1021/ja3073808
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.