Immunosensing using a metal clad leaky waveguide biosensor for clinical diagnosis

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Title
Immunosensing using a metal clad leaky waveguide biosensor for clinical diagnosis
Author(s)
Won Joo Im; Bo Bae Kim; Ju Young Byun; H M Kim; M G Kim; Yong Beom Shin
Bibliographic Citation
Sensors and Actuators B-Chemical, vol. 173, pp. 288-294
Publication Year
2012
Abstract
The optical detection technologies have been used in biosensors for the highly sensitive, real-time and label-free detection of biomolecules. In this report, a metal clad leaky waveguide (MCLW) was used as an immunosensing tools. We optimized the structure of the sensor chip for highly sensitive detection, followed by fabrication of the MCLW sensor chips and construction of a detection system for immunosensing. Titanium and SiO 2 films were deposited onto a BK7 substrate using an E-beam evaporator and the thickness of the films was 9 nm and 347 nm, respectively. The sensing response of the system to the bulk refractive index was calibrated using various concentrations of a glycerol solution. As a result, the MCLW sensor system was able to detect a change of ∼3.8 × 10 -6 RIU in the refractive index of the ambient solution. In addition, immunosensing was achieved using the MCLW sensor for an antigen-antibody reaction in real time. The biotin-labeled antibody of human interleukin 5 (hIL5) was immobilized on the sensor surface containing modified with streptavidin. The hIL5 in a bio-solution was quantitatively analyzed in real time using the MCLW sensor system.
Keyword
ImmunosensingMetal cladBiosensorOptical waveguide
ISSN
0925-4005
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.snb.2012.06.085
Type
Article
Appears in Collections:
Critical Diseases Diagnostics Convergence Research Center > 1. Journal Articles
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
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