CRP detection from serum for chip-based point-of-care testing system

Cited 41 time in scopus
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Title
CRP detection from serum for chip-based point-of-care testing system
Author(s)
C H Kim; J H Ahn; J Y Kim; J M Choi; K C Lim; T J Park; N S Heo; Hee Gu Lee; J W Kim; Y K Choi
Bibliographic Citation
Biosensors & Bioelectronics, vol. 41, no. 1, pp. 322-327
Publication Year
2013
Abstract
Most of point-of-care testing (POCT) to improve facilitates in diagnosis, treatment, and monitoring of patients. POCT technique has still remained a quantitatively and accurately detective effect. In this article, we demonstrated that real human C-reactive protein (CRP) in serum was detected for a chip-based point-of-care testing application based on a nanogap-embedded field effect transistor (FET), and the results were compared with those obtained via the enzyme-linked immunosorbent assay (ELISA) method. The limit of detection (LOD), determined from the standard curve, was 0.1. ng/ml, which is comparable to that of commercialized ELISAs. We evaluated that an improved detection range (0.1. ng/ml to 100. ng/ml) was achieved by comparing with commercialized ELISA. Control experiments to determine selectivity and to discern false-positive/false-negative rates were also performed. This report is the first description of the detection of CRP in human serum using a silicon-based biosensor.
Keyword
C-reactive proteinCRPFET-based biosensorNanogap-embedded FETPoint-of-care testing (POCT)
ISSN
0956-5663
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bios.2012.08.047
Type
Article
Appears in Collections:
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
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