Human leukocytes regulate ganglioside expression in cultured micro-pig aortic endothelial cells = 미니돼지의 대동맥 내피세포를 이용한 갱글리오 사이드의 발현 분석
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- Title
- Human leukocytes regulate ganglioside expression in cultured micro-pig aortic endothelial cells = 미니돼지의 대동맥 내피세포를 이용한 갱글리오 사이드의 발현 분석
- Author(s)
- J H Cho; Ji Su Kim; M U Lim; H K Min; D H Kwak; J S Ryu; J T Lee; Sun-Uk Kim; C H Kim; C H Kim; D B Koo; Kyu Tae Chang; Y K Choo
- Bibliographic Citation
- Laboratory Animal Research, vol. 28, no. 4, pp. 255-263
- Publication Year
- 2012
- Abstract
- Gangliosides are ubiquitous components of the membranes of mammalian cells that are thought to play
important roles in various cell functions such as cell-cell interaction, cell adhesion, cell differentiation,
growth control, and signaling. However, the role that gangliosides play in the immune rejection response
after xenotransplantation is not yet clearly understood. In this study, the regulatory effects of human
leukocytes on ganglioside expression in primary cultured micro-pig aortic endothelial cells (PAECs) were
investigated. To determine the impact of human leukocytes on the expression of gangliosides in PAECs,
we performed high-performance thin layer chromatography (HPTLC) in PAECs incubated with FBS, FBS
containing human leukocytes, human serum containing human leukocytes, and FBS containing TNF-α.
Both HPTLC and immunohistochemistry analyses revealed that PAECs incubated with FBS predominantly
express the gangliosides GM3, GM1, and GD3. However, the expression of GM1 significantly decreased
in PAECs incubated for 5 h with TNF-α (10 ng/mL), 10% human serum containing human leukocytes, and
10% FBS containing human leukocytes. Taken together, these results suggest that human leukocytes
induced changes in the expression profile of ganglioside GM1 similar to those seen upon treatment of
PAECs with TNF-α. This finding may be relevant for designing future therapeutic strategies intended to
prolong xenograft survival.
- Keyword
- Micro-pig aortic endothelial cellshuman serumhuman leukocytetumor necrosis factor-αganglioside GM1
- ISSN
- 1738-6055
- Publisher
- Springer-BMC
- DOI
- http://dx.doi.org/10.5625/lar.2012.28.4.255
- Type
- Article
- Appears in Collections:
- Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
Ochang Branch Institute > Division of National Bio-Infrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
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