Human leukocytes regulate ganglioside expression in cultured micro-pig aortic endothelial cells = 미니돼지의 대동맥 내피세포를 이용한 갱글리오 사이드의 발현 분석

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Title
Human leukocytes regulate ganglioside expression in cultured micro-pig aortic endothelial cells = 미니돼지의 대동맥 내피세포를 이용한 갱글리오 사이드의 발현 분석
Author(s)
J H Cho; Ji Su Kim; M U Lim; H K Min; D H Kwak; J S Ryu; J T Lee; Sun-Uk Kim; C H Kim; C H Kim; D B Koo; Kyu Tae Chang; Y K Choo
Bibliographic Citation
Laboratory Animal Research, vol. 28, no. 4, pp. 255-263
Publication Year
2012
Abstract
Gangliosides are ubiquitous components of the membranes of mammalian cells that are thought to play important roles in various cell functions such as cell-cell interaction, cell adhesion, cell differentiation, growth control, and signaling. However, the role that gangliosides play in the immune rejection response after xenotransplantation is not yet clearly understood. In this study, the regulatory effects of human leukocytes on ganglioside expression in primary cultured micro-pig aortic endothelial cells (PAECs) were investigated. To determine the impact of human leukocytes on the expression of gangliosides in PAECs, we performed high-performance thin layer chromatography (HPTLC) in PAECs incubated with FBS, FBS containing human leukocytes, human serum containing human leukocytes, and FBS containing TNF-α. Both HPTLC and immunohistochemistry analyses revealed that PAECs incubated with FBS predominantly express the gangliosides GM3, GM1, and GD3. However, the expression of GM1 significantly decreased in PAECs incubated for 5 h with TNF-α (10 ng/mL), 10% human serum containing human leukocytes, and 10% FBS containing human leukocytes. Taken together, these results suggest that human leukocytes induced changes in the expression profile of ganglioside GM1 similar to those seen upon treatment of PAECs with TNF-α. This finding may be relevant for designing future therapeutic strategies intended to prolong xenograft survival.
Keyword
Micro-pig aortic endothelial cellshuman serumhuman leukocytetumor necrosis factor-αganglioside GM1
ISSN
1738-6055
Publisher
Springer-BMC
DOI
http://dx.doi.org/10.5625/lar.2012.28.4.255
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Primate Resources Center > 1. Journal Articles
Ochang Branch Institute > Division of National Bio-Infrastructure > Futuristic Animal Resource & Research Center > 1. Journal Articles
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