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- Title
- Effect of porcine follicular fluid on donor cell characteristics and quality of porcine cloned blastocysts
- Author(s)
- D J Kwon; K B Oh; S A Ock; Jeong Woong Lee; S S Lee; J K Park; W K Chang; S Hwang
- Bibliographic Citation
- Reproductive & Developmental Biology, vol. 36, no. 4, pp. 249-254
- Publication Year
- 2012
- Abstract
- This study aimed at investigating whether a porcine follicular fluid (pFF) supplementation positively affects the
characteristics of donor cells and the developmental competence of porcine cloned embryos. Ear fibroblast cells (donor
cell) from an Massachusetts General Hospital miniature pig were cultured in different culture methods: (1) Dulbecco's
modified Eagle's medium (DMEM)+10% FBS (Control); (2) DMEM+0.5% FBS (SS); and (3) DMEM+10% FBS+10% pFF
(pFF) for 72 h. In each conditioned medium, the concentrations of 4 amino acids (Thr, Glu, Pro, and Val) in the pFF
group were significantly different from those in the control group (p<0.05 or p<0.01). The proliferation of the cells
cultured in the SS group was significantly lower than that of the other treatment groups (p<0.01). The population of
apoptotic and necrotic cells in the SS group was significantly higher than that of either the control or the pFF group
(p<0.01). The number of embryos that cleaved (p<0.05) and developed into blastocysts (p<0.01) in the SS group was
significantly lower than that of either the control or the pFF group. Compared to other groups, the blastocysts
produced from the donor cells in the pFF group had higher total cells and lower apoptotic cells (p<0.05). It can be
concluded that pFF supplementation in the donor cell culture medium positively affects cell death, cell cycle and
quality of the cloned blastocyst.
- Keyword
- Porcine follicular fluidAmino acidsCell cycleCloned embryo development
- ISSN
- I000-0006
- Publisher
- Korea Soc-Assoc-Inst
- Type
- Article
- Appears in Collections:
- Division of Biomedical Research > Biotherapeutics Translational Research Center > 1. Journal Articles
- Files in This Item:
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