Lipid droplet synthesis is limited by acetate availability in starchless mutant of Chlamydomonas reinhardtii

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Title
Lipid droplet synthesis is limited by acetate availability in starchless mutant of Chlamydomonas reinhardtii
Author(s)
R Ramanan; Byung-Hyuk Kim; Dae-Hyun ChoSo Ra KoHee-Mock OhHee-Sik Kim
Bibliographic Citation
FEBS Letters, vol. 587, no. 4, pp. 370-377
Publication Year
2013
Abstract
Phenotypic and genotypic changes in Chlamydomonas reinhardtii BafJ5, a starchless mutant, with respect to lipid metabolism was studied in different trophic states under nitrogen (N) sufficient and limited conditions. Interestingly, cellular lipid content increased linearly with input acetate concentration with highest lipid content (∼42%) under nitrogen limitation and mixotrophic state. RT-qPCR studies indicate that key fatty acid biosynthesis genes are down-regulated under N limitation but not under mixotrophic state, whereas, ACS2, encoding Acetyl-CoA synthetase, and DGTT4, encoding Diacylglycerol O-acyltransferase, are up-regulated under all conditions. These results collectively indicate that acetate is the limiting factor and central molecule in lipid droplet synthesis. The study also provides further evidence of the presence of a chloroplast pathway for triacylglycerol synthesis in microalgae.
Keyword
Chlamydomonas reinhardtiiChloroplastFatty acid biosynthesisGene expressionLipid droplet
ISSN
0014-5793
Publisher
Wiley
DOI
http://dx.doi.org/10.1016/j.febslet.2012.12.020
Type
Article
Appears in Collections:
Synthetic Biology and Bioengineering Research Institute > Cell Factory Research Center > 1. Journal Articles
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