Efficient myogenic differentiation of human adipose-derived stem cells by the transduction of engineered MyoD protein

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Title
Efficient myogenic differentiation of human adipose-derived stem cells by the transduction of engineered MyoD protein
Author(s)
Min Sun Sung; Ji Young Mun; Oh Suk KwonKi Sun KwonDoo-Byoung Oh
Bibliographic Citation
Biochemical and Biophysical Research Communications, vol. 437, no. 1, pp. 156-161
Publication Year
2013
Abstract
Human adipose-derived stem cells (hASCs) have great potential as cell sources for the treatment of muscle disorders. To provide a safe method for the myogenic differentiation of hASCs, we engineered the MyoD protein, a key transcription factor for myogenesis. The engineered MyoD (MyoD-IT) was designed to contain the TAT protein transduction domain for cell penetration and the membrane-disrupting INF7 peptide, which is an improved version of the HA2 peptide derived from influenza. MyoD-IT showed greatly improved nuclear targeting ability through an efficient endosomal escape induced by the pH-sensitive membrane disruption of the INF7 peptide. By applying MyoD-IT to a culture, hASCs were efficiently differentiated into long spindle-shaped myogenic cells expressing myosin heavy chains. Moreover, these cells differentiated by an application of MyoD-IT fused to myotubes with high efficiency through co-culturing with mouse C2C12 myoblasts. Because internalized proteins can be degraded in cells without altering the genome, the myogenic differentiation of hASCs using MyoD-IT would be a safe and clinically applicable method.
Keyword
Endosomal escapeHuman adipose-derived stem cellsMyoDMyogenic differentiationProtein transduction domain
ISSN
0006-291X
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.bbrc.2013.06.058
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Division of Research on National Challenges > Aging Research Center > 1. Journal Articles
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