Characterization of a novel steviol-producing beta-glucosidase from Penicillum decumbens and optimal production of the steviol = Penicillum decumbens로부터 스테비올을 생산하는 글루코시다제의 특성 및 스테비올의 대량생산

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Title
Characterization of a novel steviol-producing beta-glucosidase from Penicillum decumbens and optimal production of the steviol = Penicillum decumbens로부터 스테비올을 생산하는 글루코시다제의 특성 및 스테비올의 대량생산
Author(s)
Jin A Ko; Young Bae RyuHyung Jun KwonHyung Jae JeongSu-Jin ParkCha Young Kim; Y J Wee; D Kim; Woo Song Lee; Young-Min Kim
Bibliographic Citation
Applied Microbiology and Biotechnology, vol. 97, no. 18, pp. 8151-8161
Publication Year
2013
Abstract
This study aimed to develop an economically viable enzyme for the optimal production of steviol (S) from stevioside (ST). Of 9 commercially available glycosidases tested, S-producing β-glucosidase (SPGase) was selected and purified 74-fold from Penicillium decumbens naringinase by a three-step column chromatography procedure. The 121-kDa protein was stable at pH 2.3-6.0 and at 40-60 C. Hydrolysis of ST by SPGase produced rubusoside (R), steviolbioside (SteB), steviol mono-glucoside (SMG), and S, as determined by HPLC, HPLC-MS, and 1H- and 13C-nuclear magnetic resonance. SPGase showed higher activity toward steviol mono-glucosyl ester, ST, R, and SMG than other β-linked glucobioses. The optimal conditions for S production (30 mM, 64 % yield) were 47 mM ST and 43 μl of SPGase at pH 4.0 and 55 C. This is the first report detailing the production of S from ST hydrolysis by a novel β-glucosidase, which may be useful for the pharmaceutical and agricultural areas.
Keyword
β-GlucosidaseNaringinasePenicillium decumbensSteviolStevioside
ISSN
0175-7598
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s00253-013-4883-0
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
Jeonbuk Branch Institute > 1. Journal Articles
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