RecA-mediated SOS response provides a geraniol tolerance in Escherichia coli

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Title
RecA-mediated SOS response provides a geraniol tolerance in Escherichia coli
Author(s)
A A Shah; C Wang; S H Yoon; J Y Kim; Eui Sung Choi; S W Kim
Bibliographic Citation
Journal of Biotechnology, vol. 167, no. 4, pp. 357-364
Publication Year
2013
Abstract
Geraniol is an important industrial material and a potential candidate of advanced biofuels. One challenge of microbial geraniol production is the toxicity to hosts. However, the poor understanding on geraniol tolerance mechanism is an obstacle for developing geraniol tolerant host. This study genome-widely screened a shot-gun DNA library of Escherichia coli and found that recA is able to confer geraniol tolerance in E. coli. The recA knockout mutant was found extremely sensitive to geraniol. Based on our data, it was deciphered that recA provided tolerance through SOS response network responding to DNA damage caused by geraniol. RecA-mediated SOS response activates the homologous recombinational repair by RecB and RecN for corrective DNA maintenance. This protection mechanism suggests an effective strategy to combat geraniol toxicity in E. coli.
Keyword
DNA repairEscherichia coliGeraniolRecASOS responseStress tolerance
ISSN
0168-1656
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.jbiotec.2013.07.023
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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