Activity-guided screening of anti-inflammatory compounds from the hexane extracts of Schisandra chinensis fruit = 생리활성분획 추적방법을 통한 오미자 추출물의 항염증 활성 분석

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Title
Activity-guided screening of anti-inflammatory compounds from the hexane extracts of Schisandra chinensis fruit = 생리활성분획 추적방법을 통한 오미자 추출물의 항염증 활성 분석
Author(s)
H J Choi; Y W Choi; S Y Baek; B S Kim; S C Ahn; Moon Soo Rhee; S Yoon
Bibliographic Citation
Journal of Life Science, vol. 23, no. 2, pp. 311-318
Publication Year
2013
Abstract
Schisandra chinensis containing a variety of pharmacologically active lignans has been traditionally used in oriental medicine. In this study, anti-inflammatory compounds were screened from the hexane extracts of S. chinensis by activity-guided fractionation. First, we investigated the regulatory effects on the expression of E-selectin, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) with 38 fractions from the hexane extracts of S. chinensis in human umbilical vein endothelial cells (HUVECs). As a result, SCKH1 among the 38 fractions from the hexane extract of S. chinensis was selected for further analysis based on its unique regulatory effect on cell adhesion molecules, especially on VCAM-1, in LPS-stimulated HUVECs. The subsequent activity-guided fractionation of SCKH1 resulted in the purification of SCKH1PAIBPB, which was found to suppress the expression of VCAM-1, MCP-1, IL-6 and IL-8 in HUVECs stimulated with LPS, and to inhibit the adhesive capacity between HUVECs and monocytes. Taken together, our data indicate that SCKH1PAIBPB can be proposed as an effective anti-inflammatory compound that may have a potential therapeutic use for the prevention and treatment of various inflammatory diseases as well as ischemic vascular diseases.
Keyword
Activity-guided isolationanti-inflammatorycell adhesion moleculeSchisandra chinensisSCKH1PAIBPB
ISSN
I000-0128
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5352/JLS.2013.23.2.311
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Biological Resource Center > 1. Journal Articles
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