Engineering Escherichia coli for selective geraniol production with minimized endogenous dehydrogenation

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Title
Engineering Escherichia coli for selective geraniol production with minimized endogenous dehydrogenation
Author(s)
J Zhou; C Wang; S H Yoon; H J Jang; Eui Sung Choi; S W Kim
Bibliographic Citation
Journal of Biotechnology, vol. 169, no. 1, pp. 42-50
Publication Year
2014
Abstract
Geraniol, a monoterpene alcohol, has versatile applications in the fragrance industry, pharmacy and agrochemistry. Moreover, geraniol could be an ideal gasoline alternative. In this study, recombinant overexpression of geranyl diphosphate synthase and the bottom portion of a foreign mevalonate pathway in Escherichia coli MG1655 produced 13.3. mg/L of geraniol. Introduction of Ocimum basilicum geraniol synthase increased geraniol production to 105.2. mg/L. However, geraniol production encountered a loss from its endogenous dehydrogenization and isomerization into other geranoids (nerol, neral and geranial). Three E. coli enzymes (YjgB, YahK and YddN) were identified with high sequence identity to plant geraniol dehydrogenases. YjgB was demonstrated to be the major one responsible for geraniol dehydrogenization. Deletion of yjgB increased geraniol production to 129.7. mg/L. Introduction of the whole mevalonate pathway for enhanced building block synthesis from endogenously synthesized mevalonate improved geraniol production up to 182.5. mg/L in the yjgB mutant after 48. h of culture, which was a double of that obtained in the wild type control (96.5. mg/L). Our strategy for improving geraniol production in engineered E. coli should be generalizable for addressing similar problems during metabolic engineering.
Keyword
E. coliGeraniolGeraniol dehydrogenationMevalonate pathwayMonoterpene
ISSN
0168-1656
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.jbiotec.2013.11.009
Type
Article
Appears in Collections:
Division of Bio Technology Innovation > BioProcess Engineering Center > 1. Journal Articles
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