Isolation and functional characterization of a delta 6-desaturase gene from the pike eel (Muraenesox cinereus)

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Title
Isolation and functional characterization of a delta 6-desaturase gene from the pike eel (Muraenesox cinereus)
Author(s)
S H Kim; K H Roh; J B Kim; K S Kim; Namshin Kim; H U Kim; K R Lee; J S Park; J B Kim
Bibliographic Citation
Journal of Microbiology, vol. 51, no. 6, pp. 807-813
Publication Year
2013
Abstract
Stearidonic acid (STA; 18:4n-3) and γ-linolenic acid (GLA; 18:3n-6) are significant intermediates in the biosynthetic pathway for the very-long-chain polyunsaturated fatty acids of eicosapentaenoic acid (EPA; 20:5n-3) and arachidonic acid (ARA; 20:4n-6), respectively. To develop a sustainable system for the production of dietary polyunsaturated fatty acids, we focused on the action of the enzyme delta 6-desaturase (D6DES) on the essential acids, linoleic acid (LA; 18:2n-6) and α-linolenic acid (ALA; 18:3n-3). A 1,335-bp full-length cDNA encoding D6DES (McD6DES) was cloned from Muraenesox cinereus using degenerate PCR and RACE-PCR methods. To investigate the enzymatic activity of McD6DES in the production of n-6 and n-3 fatty acids, a recombinant plasmid expressing McD6DES (pYES-McD6DES) was transformed into and expressed in Saccharomyces cerevisiae. The exogenously expressed McD6DES produced GLA and STA at conversion rates of 14.2% and 45.9%, respectively, from the exogenous LA and ALA substrates. These results indicate that McD6DES is essentially a delta 6-desaturase involved in very-long-chain polyunsaturated fatty acid synthesis
Keyword
Γ-linolenic aciddelta 6-desaturaseMuraenesox cinereusSaccharomyces cerevisiaestearidonic acid
ISSN
1225-8873
Publisher
Microbiological Society Korea
DOI
http://dx.doi.org/10.1007/s12275-013-3144-3
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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