Aralia cordata inhibits triacylglycerol biosynthesis in HepG2 cells

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Title
Aralia cordata inhibits triacylglycerol biosynthesis in HepG2 cells
Author(s)
Mun-Ock Kim; S H Lee; J H Seo; I S Kim; Ah Reum Han; D O Moon; Sungchan Cho; L Cui; J Kim; Hyun Sun Lee
Bibliographic Citation
Journal of Medicinal Food, vol. 16, no. 12, pp. 1108-1114
Publication Year
2013
Abstract
Glycerol-3-phosphate acyltransferase (GPAT) catalyzes the first committed step in triacylglycerol (TAG) and phospholipid biosynthesis, and has been considered as one of the drug targets for treating hepatic steatosis, insulin resistance, and other metabolic disorders. The aim of this study was to investigate the GPAT inhibitors from natural products and to evaluate their effects. The methanol extract of Aralia cordata roots showed a strong inhibitory effect on the human GPAT1 activity. A further bioactivity-guided approach led to the isolation of ent-pimara-8(14),15-dien-19-oic acid, (PA), one of the major compounds of A. cordata, which suppressed the GPAT1 activity with IC 50 value of 60.5 μM. PA markedly reduced de novo lysophosphatidic acid synthesis through inhibition of GPAT activity and therefore significantly decreased synthesis of TAG in the HepG2 cells. These results suggest that PA as well as A. cordata root extract could be beneficial in controlling lipid metabolism.
Keyword
Aralia cordataEnt-pimara-8(14),15-dien-19-oic acidGlycerol-3-phosphate acyltransferaseLysophophatidic acidTriacylglycerol
ISSN
1096-620X
Publisher
Mary Ann Liebert, Inc
DOI
http://dx.doi.org/10.1089/jmf.2012.2636
Type
Article
Appears in Collections:
Ochang Branch Institute > Natural Medicine Research Center > 1. Journal Articles
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