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- Title
- Aralia cordata inhibits triacylglycerol biosynthesis in HepG2 cells
- Author(s)
- Mun-Ock Kim; S H Lee; J H Seo; I S Kim; Ah Reum Han; D O Moon; Sungchan Cho; L Cui; J Kim; Hyun Sun Lee
- Bibliographic Citation
- Journal of Medicinal Food, vol. 16, no. 12, pp. 1108-1114
- Publication Year
- 2013
- Abstract
- Glycerol-3-phosphate acyltransferase (GPAT) catalyzes the first committed step in triacylglycerol (TAG) and phospholipid biosynthesis, and has been considered as one of the drug targets for treating hepatic steatosis, insulin resistance, and other metabolic disorders. The aim of this study was to investigate the GPAT inhibitors from natural products and to evaluate their effects. The methanol extract of Aralia cordata roots showed a strong inhibitory effect on the human GPAT1 activity. A further bioactivity-guided approach led to the isolation of ent-pimara-8(14),15-dien-19-oic acid, (PA), one of the major compounds of A. cordata, which suppressed the GPAT1 activity with IC 50 value of 60.5 μM. PA markedly reduced de novo lysophosphatidic acid synthesis through inhibition of GPAT activity and therefore significantly decreased synthesis of TAG in the HepG2 cells. These results suggest that PA as well as A. cordata root extract could be beneficial in controlling lipid metabolism.
- Keyword
- Aralia cordataEnt-pimara-8(14),15-dien-19-oic acidGlycerol-3-phosphate acyltransferaseLysophophatidic acidTriacylglycerol
- ISSN
- 1096-620X
- Publisher
- Mary Ann Liebert, Inc
- DOI
- http://dx.doi.org/10.1089/jmf.2012.2636
- Type
- Article
- Appears in Collections:
- Ochang Branch Institute > Natural Product Research Center > 1. Journal Articles
Ochang Branch Institute > Nucleic Acid Therapeutics Research Center > 1. Journal Articles
- Files in This Item:
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