Optimized production of 2,3-butanediol by a lactate dehydrogenase-deficient mutant of Klebsiella pneumoniae = Lactate dehydrogenase 결손 Klebsiella pneumoniae를 사용한 2,3-Butanediol 최적 생산

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Title
Optimized production of 2,3-butanediol by a lactate dehydrogenase-deficient mutant of Klebsiella pneumoniae = Lactate dehydrogenase 결손 Klebsiella pneumoniae를 사용한 2,3-Butanediol 최적 생산
Author(s)
S B Lee; Baek Rock Oh; Jang Min Park; A Yu; Sun-Yeon Heo; Won-Kyung Hong; Jeong-Woo SeoChul Ho Kim
Bibliographic Citation
Biotechnology and Bioprocess Engineering, vol. 18, pp. 1210-1215
Publication Year
2013
Abstract
To obtain high-yield production of 2,3-butanediol (2,3-BD) from glucose, we optimized the culture conditions for a lactate dehydrogenase-deficient mutant (ΔldhA) of Klebsiella pneumoniae using response surface methodology. 2,3-BD production was successfully improved by optimizing pH (5.6), aeration (3.50 vvm) and concentration of corn steep liquor (45.0 mL/L) as a nitrogen source, resulting in a maximum level of 2,3-BD production of 148.8 g/L and productivity of 2.48 g/L/h. 2,3-BD was also obtained with high concentration (76.24 g/L) and productivity (2.31 g/L/h) from the K. pneumoniae mutant strain using sugarcane molasses as a carbon source.
Keyword
2,3-butanediolKlebsiella pneumoniaelactate dehydrogenasedeficient mutantresponse surface methodologysugarcane molasses
ISSN
1226-8372
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s12257-013-0396-z
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
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