Development of the large-scale oligonucleotide chip for the diagnosis of plant viruses and its practical use

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Title
Development of the large-scale oligonucleotide chip for the diagnosis of plant viruses and its practical use
Author(s)
M Nam; J S Kim; Seungmo Lim; C Y Park; J G Kim; H S Choi; H S Lim; Jae Sun Moon; S H Lee
Bibliographic Citation
Plant Pathology Journal, vol. 30, no. 1, pp. 51-57
Publication Year
2014
Abstract
A large-scale oligonucleotide (LSON) chip was developed for the detection of the plant viruses with known genetic information. The LSON chip contains two sets of 3,978 probes for 538 species of targets including plant viruses, satellite RNAs and viroids. A hundred forty thousand probes, consisting of isolate-, species- and genus-specific probes respectively, are designed from 20,000 of independent nucleotide sequence of plant viruses. Based on the economic importance, the amount of genome information, and the number of strains and/or isolates, one to fifty-one probes for each target virus are selected and spotted on the chip. The standard and field samples for the analysis of the LSON chip have been prepared and tested by RT-PCR. The probe's specific and/or nonspecific reaction patterns by LSON chip allow us to diagnose the unidentified viruses. Thus, the LSON chip in this study could be highly useful for the detection of unexpected plant viruses, the monitoring of emerging viruses and the fluctuation of the population of major viruses in each plant.
Keyword
Large-scale oligonucleotide chipLSON chipOligo chipPlant virusVirus diagnosis
ISSN
I000-0113
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.5423/PPJ.OA.08.2013.0084
Type
Article
Appears in Collections:
Division of Research on National Challenges > Plant Systems Engineering Research > 1. Journal Articles
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