Expression of Dengue virus EIII domain-coding gene in maize as an edible vaccine candidate

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Title
Expression of Dengue virus EIII domain-coding gene in maize as an edible vaccine candidate
Author(s)
Hyun A Kim; Suk Yoon Kwon; M S Yang; P S Choi
Bibliographic Citation
Journal of Plant Biotechnology, vol. 41, no. 1, pp. 50-55
Publication Year
2014
Abstract
Plant-based vaccines possess some advantages over other types of vaccine biotechnology such as safety, low cost of mass vaccination programs, and wider use of vaccines for medicine. This study was undertaken to develop the transgenic maize as edible vaccine candidates for humans. The immature embryos of HiII genotype were inoculated with A. tumefaciens strain C58C1 containing the binary vectors (V662 or V663). The vectors carrying nptII gene as selection marker and scEDIII (V662) or wCTB-scEDIII (V663) target gene, which code EIII proteins inhibite viral adsorption by cells. In total, 721 maize immature embryos were transformed and twenty-two putative transgenic plants were regenerated after 12 weeks selection regime. Of them, two- and six-plants were proved to be integrated with scEDIII and wCTB-scEDIII genes, respectively, by Southern blot analysis. However, only one plant (V662-29-3864) can express the gene of interest confirmed by Northern blot analysis. These results demonstrated that this plant could be used as a candidated source of the vaccine production.
Keyword
Dengue virusEdible vaccineImmature embryoMaize transformationParomomycin
ISSN
1229-2818
Publisher
South Korea
DOI
http://dx.doi.org/10.5010/JPB.2014.41.1.50
Type
Article
Appears in Collections:
Division of Biomaterials Research > 1. Journal Articles
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