RNA-guided genome editing in Drosophila with the purified Cas9 protein

Cited 37 time in scopus
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Title
RNA-guided genome editing in Drosophila with the purified Cas9 protein
Author(s)
Jeong Soo Lee; Su-Jin Kwak; J Kim; Ae Kyung Kim; Hae Min Noh; J S Kim; Kweon Yu
Bibliographic Citation
G3-Genes Genomes Genetics, vol. 4, no. 7, pp. 1291-1295
Publication Year
2014
Abstract
We report a method for generating Drosophila germline mutants effectively via injection of the complex of the purified Cas9 protein, tracrRNA, and gene-specific crRNAs, which may reduce delayed mutations because of the transient activity of the Cas9 protein, combined with the simple mutation detection in GO founders by the T7E1 assay.
Keyword
DrosophilaRGENCas9 protein
ISSN
2160-1836
Publisher
Genetics Society America
DOI
http://dx.doi.org/10.1534/g3.114.012179
Type
Article
Appears in Collections:
Division of Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
Ochang Branch Institute > Division of National Bio-Infrastructure > 1. Journal Articles
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