Open Access@KRIBBDivision of A.I. & Biomedical ResearchMicrobiome Convergence Research Center1. Journal Articles
RNA-guided genome editing in Drosophila with the purified Cas9 protein
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- Title
- RNA-guided genome editing in Drosophila with the purified Cas9 protein
- Author(s)
- Jeong Soo Lee; Su-Jin Kwak; J Kim; Ae Kyung Kim; Hae Min Noh; J S Kim; Kweon Yu
- Bibliographic Citation
- G3-Genes Genomes Genetics, vol. 4, no. 7, pp. 1291-1295
- Publication Year
- 2014
- Abstract
- We report a method for generating Drosophila germline mutants effectively via injection of the complex of the purified Cas9 protein, tracrRNA, and gene-specific crRNAs, which may reduce delayed mutations because of the transient activity of the Cas9 protein, combined with the simple mutation detection in GO founders by the T7E1 assay.
- Keyword
- DrosophilaRGENCas9 protein
- ISSN
- 2160-1836
- Publisher
- Genetics Society America
- Full Text Link
- http://dx.doi.org/10.1534/g3.114.012179
- Type
- Article
- Appears in Collections:
- Division of A.I. & Biomedical Research > Microbiome Convergence Research Center > 1. Journal Articles
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
Ochang Branch Institute > Division of National Bio-Infrastructure > 1. Journal Articles
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