Monitoring the differentiation and migration patterns of neural cells derived from human embryonic stem cells using a microfluidic culture system

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Title
Monitoring the differentiation and migration patterns of neural cells derived from human embryonic stem cells using a microfluidic culture system
Author(s)
N Lee; J W Park; H J Kim; J H Yeon; J Kwon; J J Ko; S H Oh; H S Kim; A Kim; Baek Soo HanSang Chul Lee; N L Jeon; J Song
Bibliographic Citation
Molecules and Cells, vol. 37, no. 6, pp. 497-502
Publication Year
2014
Abstract
Microfluidics can provide unique experimental tools to visualize the development of neural structures within a microscale device, which is followed by guidance of neurite growth in the axonal isolation compartment. We utilized microfluidics technology to monitor the differentiation and migration of neural cells derived from human embryonic stem cells (hESCs). We co-cultured hESCs with PA6 stromal cells, and isolated neural rosette-like structures, which subsequently formed neurospheres in suspension culture. Tuj1-positive neural cells, but not nestin-positive neural precursor cells (NPCs), were able to enter the microfluidics grooves (microchannels), suggesting that neural cell-migratory capacity was dependent upon neuronal differentiation stage. We also showed that bundles of axons formed and extended into the microchannels. Taken together, these results demonstrated that microfluidics technology can provide useful tools to study neurite outgrowth and axon guidance of neural cells, which are derived from human embryonic stem cells.
Keyword
AxonsHuman embryonic stem cellsMicrofluidicsMigrationNeural differentiation
ISSN
1016-8478
Publisher
South Korea
DOI
http://dx.doi.org/10.14348/molcells.2014.0137
Type
Article
Appears in Collections:
Division of Research on National Challenges > Biodefense Research Center > 1. Journal Articles
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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