Identification of lactoferrin as a human dedifferentiation factor through the studies of reptile tissue regeneration mechanisms

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Title
Identification of lactoferrin as a human dedifferentiation factor through the studies of reptile tissue regeneration mechanisms
Author(s)
K S Bae; Sun Young Kim; S Y Park; A J Jeong; H H Lee; Jungwoon LeeYee Sook Cho; S H Leem; T H Kang; Kwang-Hee Bae; J H Kim; Y W Jung; W Jun; Suk Ran YoonSang Chul Lee; J W Chung
Bibliographic Citation
Journal of Microbiology and Biotechnology, vol. 24, no. 6, pp. 869-878
Publication Year
2014
Abstract
In this study, we performed two-dimensional electrophoresis with protein extracts from lizard tails, and analyzed the protein expression profiles during the tissue regeneration to identify the dedifferentiation factor. As a result, we identified 18 protein spots among total of 292 spots, of which proteins were specifically expressed during blastema formation. We selected lactoferrin as a candidate because it is the mammalian homolog of leech-derived tryptase inhibitor, which showed the highest frequency among the 18 proteins. Lactoferrin was specifically expressed in various stem cell lines, and enhanced the efficiency of iPSC generation upto approximately 7-fold relative to the control. Furthermore, lactoferrin increased the efficiency by 2-fold without enforced expression of Klf4. These results suggest that lactoferrin may induce dedifferentiation, at least partly by increasing the expression of Klf4.
Keyword
DedifferentiationIPSCsLactoferrinStem cellTissue regeneration
ISSN
1017-7825
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.4014/jmb.1402.02009
Type
Article
Appears in Collections:
Division of Research on National Challenges > Environmental diseases research center > 1. Journal Articles
Division of Biomedical Research > Immunotherapy Research Center > 1. Journal Articles
Division of Biomedical Research > Metabolic Regulation Research Center > 1. Journal Articles
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