Production of rubusoside from stevioside by using a thermostable lactase from Thermus thermophilus and solubility enhancement of liquiritin and teniposide

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Title
Production of rubusoside from stevioside by using a thermostable lactase from Thermus thermophilus and solubility enhancement of liquiritin and teniposide
Author(s)
T T H Nguyen; S J Jung; H K Kang; Young-Min Kim; Y H Moon; M Kim; D Kim
Bibliographic Citation
Enzyme and Microbial Technology, vol. 64, no. 1, pp. 38-43
Publication Year
2014
Abstract
Solubility is an important factor for achieving the desired plasma level of drug for pharmacological response. About 40% of drugs are not soluble in water in practice and therefore are slowly absorbed, which results in insufficient and uneven bioavailability and GI toxicity. Rubusoside (Ru) is a sweetener component in herbal tea and was discovered to enhance the solubility of a number of pharmaceutically and medicinally important compounds, including anticancer compounds. In this study, thirty-one hydrolyzing enzymes were screened for the conversion of stevioside (Ste) to Ru. Recombinant lactase from Thermus thermophiles which was expressed in Escherichia coli converted stevioside to rubusoside as a main product. Immobilized lactase was prepared and used for the production of rubusoside; twelve reaction cycles were repeated with 95.4% of Ste hydrolysis and 49gL-1 of Ru was produced. The optimum rubusoside synthesis yield was 86% at 200gL-1, 1200U lactase. The purified 10% rubusoside solution showed increased water solubility of liquiritin from 0.98mgmL-1 to 4.70±0.12mgmL-1 and 0mgmL-1 to 3.42±0.11mgmL-1 in the case of teniposide
Keyword
Immobilized lactaseLiquiritinRubusosideTeniposideThermus thermophilus
ISSN
0141-0229
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.enzmictec.2014.07.001
Type
Article
Appears in Collections:
1. Journal Articles > Journal Articles
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