Spectral reproducibility and quantification of peptides in MALDI of samples prepared by micro-spotting

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Title
Spectral reproducibility and quantification of peptides in MALDI of samples prepared by micro-spotting
Author(s)
Y J Bae; K M Park; S H Ahn; Jeong Hee Moon; M S Kim
Bibliographic Citation
Journal of American Society for Mass Spectrometry, vol. 25, no. 8, pp. 1502-1505
Publication Year
2014
Abstract
Previously, we reported that MALDI spectra of peptides became reproducible when temperature was kept constant. Linear calibration curves derived from such spectral data could be used for quantification. Homogeneity of samples was one of the requirements. Among the three popular matrices used in peptide MALDI [i.e., α-cyano-4-hydroxycinnamic acid (CHCA), 2,5-dihydroxybenzoic acid (DHB), and sinapinic acid (SA)], homogeneous samples could be prepared by conventional means only for CHCA. In this work, we showed that sample preparation by micro-spotting improved the homogeneity for all three cases.
Keyword
HomogeneityMALDIMicro-spottingQuantificationReproducibility
ISSN
1044-0305
Publisher
Amer Chem Soc
DOI
http://dx.doi.org/10.1007/s13361-014-0919-7
Type
Article
Appears in Collections:
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
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