Purification and functional characterization of the first stilbene glucoside-specific beta-glucosidase isolated from Lactobacillus kimchi = 락토바실러스 유래 균으로부터 새로운 스틸벤 배당화합물의 분리 및 기능성 연구

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Title
Purification and functional characterization of the first stilbene glucoside-specific beta-glucosidase isolated from Lactobacillus kimchi = 락토바실러스 유래 균으로부터 새로운 스틸벤 배당화합물의 분리 및 기능성 연구
Author(s)
Jin-A Ko; Ji Young ParkHyung Jun KwonYoung Bae RyuHyung Jae JeongSu-Jin ParkCha Young Kim; Hyun-Mee Oh; Chan Sun Park; Y H Lim; D Kim; Mun Chual RhoWoo Song Lee; Young-Min Kim
Bibliographic Citation
Enzyme and Microbial Technology, vol. 67, pp. 59-66
Publication Year
2014
Abstract
This study aimed to develop viable enzymes for bioconversion of resveratrol-glucoside into resveratrol. Out of 13 bacterial strains tested, Lactobacillus kimchi JB301 could completely convert polydatin into resveratrol. The purified enzyme had an optimum temperature of 30-40°C and optimum pH of pH 5.0 against polydatin. This enzyme showed high substrate specificities towards different substrates in the following order: isorhaponticin "polydatin" mulberroside A>oxyresveratrol-3-O-glucoside. Additionally, it rarely hydrolyzed astringin and desoxyrhaponticin. Based on these catalytic specificities, we suggest this enzyme be named stilbene glucoside-specific β-glucosidase. Furthermore, polydatin extracts from Polygonum cuspidatum were successfully converted to resveratrol with a high yield (of over 99%). Stilbene glucoside-specific β-glucosidase is the first enzyme isolated from lactic acid bacteria capable of bio-converting various stilbene glucosides into stilbene.
Keyword
BioconversionLactobacillus kimchiPolydatinResveratrolβ-Glucosidase
ISSN
0141-0229
Publisher
Elsevier
DOI
http://dx.doi.org/10.1016/j.enzmictec.2014.09.001
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Functional Biomaterial Research Center > 1. Journal Articles
Jeonbuk Branch Institute > 1. Journal Articles
Jeonbuk Branch Institute > Immunoregulatory materials Research Center > 1. Journal Articles
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