Expression and characterization of a novel 2-deoxyribose-5-phosphate aldolase from Haemophilus influenzae Rd KW20

Cited 10 time in scopus
Metadata Downloads
Title
Expression and characterization of a novel 2-deoxyribose-5-phosphate aldolase from Haemophilus influenzae Rd KW20
Author(s)
Mi-Hee Woo; Min Soo Kim; N Chung; Joong Su Kim
Bibliographic Citation
Journal of Korean Society for Applied Biological Chemistry, vol. 57, no. 5, pp. 655-660
Publication Year
2014
Abstract
A codon-optimized 2-deoxyribose-5-phosphate aldolase (DERA) gene from Haemophilus influenzae Rd KW20 was synthesized and expressed in Escherichia coli, and the biochemical properties of its product were investigated. DERA was purified using affinity chromatography and characterized using 2-deoxyribose-5-phosphate as the substrate. Specific activity of the recombinant DERA was 34.1 Umg -1. The pH and temperature optima were 7.5 and 40°C, respectively. Additionally, the recombinant enzyme retained stability up to temperatures below 50°C. Maximal enzyme activity was observed in presence of 300 mM of acetaldehyde. The apparent Km and Vmax of purified enzyme towards 2-deoxyribose-5-phosphate were 0.14 mM and 70.42 μmol min -1 mg -1 and towards 2-deoxy-D-ribose were 24.77 mM and 1.94 μmol min -1 mg -1, respectively. For synthesis of statin intermediates, the bioconversion process for production of (3R, 5S)-6-chloro-2,4,6-trideoxy-erythro-hexose from chloroacetaldehyde and acetaldehyde using the recombinant DERA was studied and this process took 3 h for maximal conversion. This recombinant DERA could be potentially applied in the production of (3R, 5S)-6-chloro-2,4,6-trideoxy-erythro-hexose.
Keyword
2-deoxyribose-5-phosphate aldolaseHaemophilus influenzaestatin intermediates synthesis
ISSN
I000-0171
Publisher
Korea Soc-Assoc-Inst
DOI
http://dx.doi.org/10.1007/s13765-014-4231-9
Type
Article
Appears in Collections:
Jeonbuk Branch Institute > Microbial Biotechnology Research Center > 1. Journal Articles
Division of Bio Technology Innovation > SME Support Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.