Smart nanoprobes for the detection of alkaline phosphatase activity during osteoblast differentiation

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Title
Smart nanoprobes for the detection of alkaline phosphatase activity during osteoblast differentiation
Author(s)
Eun Kyung Lim; Joo Oak Keem; H S Yun; Jin Young Jeong; Bong Hyun Chung
Bibliographic Citation
Chemical Communications, vol. 51, no. 15, pp. 3270-3272
Publication Year
2015
Abstract
Gold nanoparticle-conjugated fluorescent hydroxyapatite (AuFHAp) was developed as a smart nanoprobe for measuring alkaline phosphatase (ALP) activity. AuFHAp showed NIR fluorescence due to the hydrolysis of its phosphate groups by ALP. In addition, gold nanoparticles help reduce the nonspecific signal by absorbing nonspecific fluorescence. Through in vitro tests, we confirmed that the AuFHAp probe was capable of detecting ALP levels related to osteoblast activity in living cells with high fluorescence intensity.
ISSN
1359-7345
Publisher
Royal Soc Chem
DOI
http://dx.doi.org/10.1039/c4cc09620g
Type
Article
Appears in Collections:
Division of Research on National Challenges > Bionanotechnology Research Center > 1. Journal Articles
Division of Research on National Challenges > Environmental diseases research center > 1. Journal Articles
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