NgR1 expressed in P19 embryonal carcinoma cells differentiated by retinoic acid can activate STAT3

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Title
NgR1 expressed in P19 embryonal carcinoma cells differentiated by retinoic acid can activate STAT3
Author(s)
S I Lee; Ji Eun Yun; J Y Baek; Y J Jeong; Jin Ah Kim; Jong Soon Kang; S H Park; S K Kim; S K Park
Bibliographic Citation
Korean Journal of Physiology & Pharmacology, vol. 19, no. 2, pp. 105-109
Publication Year
2015
Abstract
NgR1, a Nogo receptor, is involved in inhibition of neurite outgrowth and axonal regeneration and regulation of synaptic plasticity. P19 embryonal carcinoma cells were induced to differentiate into neuron-like cells using all trans-retinoic acid and the presence and/or function of cellular molecules, such as NgR1, NMDA receptors and STAT3, were examined. Neuronally differentiated P19 cells expressed the mRNA and protein of NgR1, which could stimulate the phosphorylation of STAT3 when activated by Nogo-P4 peptide, an active segment of Nogo-66. During the whole period of differentiation, mRNAs of all of the NMDA receptor subtypes tested (NR1, NR2A-2D) were consistently expressed, which meant that neuronally differentiated P19 cells maintained some characteristics of neurons, especially central nervous system neurons. Our results suggests that neuronally differentiated P19 cells expressing NgR1 may be an efficient and convenient in vitro model for studying the molecular mechanism of cellular events that involve NgR1 and its binding partners, and for screening compounds that activate or inhibit NgR1.
Keyword
Neuronal differentiationNMDA receptorNogo receptorP19 cellSTAT3
ISSN
1226-4512
Publisher
South Korea
DOI
http://dx.doi.org/10.4196/kjpp.2015.19.2.105
Type
Article
Appears in Collections:
Ochang Branch Institute > Division of Bioinfrastructure > Laboratory Animal Resource Center > 1. Journal Articles
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