Polyethylenimine-poly(amidoamine) dendrimer modified with l-arginines as an efficient gene delivery vector

Cited 2 time in scopus
Metadata Downloads
Title
Polyethylenimine-poly(amidoamine) dendrimer modified with l-arginines as an efficient gene delivery vector
Author(s)
N Y Ahn; T H Kim; S J Song; J M Moon; Tai Hwan Ha; J S Choi
Bibliographic Citation
Macromolecular Research, vol. 23, no. 8, pp. 726-733
Publication Year
2015
Abstract
In this study, we synthesized polyethylenimine-polyamidoamine-arginine dendritic polymers (PPRs) as vectors for gene delivery. Four polymers, polyethylenimine-polyamidoamine generation 1 (PP1), PP2, PP1-arginine (PP1R), and PP2-arginine (PP2R), were synthesized and confirmed by 1H NMR. PPRs were shown to interact with and condense plasmid DNA effectively to form 171-179 nm polyplexes with 30-32 mV of zeta potentials at weight ratio 4:1 (polymer:plasmid DNA). Cytotoxicity of PPRs/pDNA complexes was lower than that of polyethylenimine (PEI) 25 kDa/pDNA complexes for all concentration ranges tested. In 293 cells, PP1R/pDNA complexes showed higher gene transfection efficiency than PEI 25 kDa. These results suggest that PPR could be promising dendritic gene carriers for gene therapy.[Figure not available: see fulltext.]
Keyword
dendrimerL-argininenanoparticlespoly(amidoamine)polyethylenimine
ISSN
1598-5032
Publisher
Polymer Soc Korea
DOI
http://dx.doi.org/10.1007/s13233-015-3101-6
Type
Article
Appears in Collections:
Division of Biomaterials Research > Bionanotechnology Research Center > 1. Journal Articles
Files in This Item:
  • There are no files associated with this item.


Items in OpenAccess@KRIBB are protected by copyright, with all rights reserved, unless otherwise indicated.