Sulfuretin induces osteoblast differentiation through activation of TGF-β signaling = TGF베타 신호전달체계를 통한 설퓨레틴의 조골세포 분화 촉진

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Title
Sulfuretin induces osteoblast differentiation through activation of TGF-β signaling = TGF베타 신호전달체계를 통한 설퓨레틴의 조골세포 분화 촉진
Author(s)
N J Song; S M Kwon; S Kim; H J Yoon; C R Seo; B Jang; S H Chang; J M Ku; Jeong Soo Lee; K M Park; J W Hong; G H Kim; K W Park
Bibliographic Citation
Molecular and Cellular Biochemistry, vol. 410, no. 1, pp. 55-63
Publication Year
2015
Abstract
The identification and examination of potential determinants controlling the progression of cell fate toward osteoblasts can be intriguing subjects. In this study, the effects of sulfuretin, a major compound isolated from Rhus verniciflua Stokes, on osteoblast differentiation were investigated. Treatments of sulfuretin induced alkaline phosphatase (ALP) activity in mesenchymal C3H10T1/2 cells and mineralization in preosteoblast MC3T3-E1 cells. Pro-osteogenic effects of sulfuretin were consistently observed in freshly isolated primary bone marrow cells. In mechanical studies, sulfuretin specifically induced expression of TGF-β target genes, such as SMAD7 and PAI-1, but not other signaling pathway-related genes. Similar to the results of gene expression analysis, reporter assays further demonstrated TGF-β-specific induction by sulfuretin. Furthermore, disruption of TGF-β signaling using treatment with TGF-β-specific inhibitor, SB-431542, and introduction of SMAD2/3 small interfering RNA impaired the effects of sulfuretin in inducing ALP activity and expression of ALP mRNA. Together, these data indicate that the pro-osteogenic effects of sulfuretin are mediated through activation of TGF-β signaling, further supporting the potential of sulfuretin in the prevention of bone-related diseases such as bone fracture and osteoporosis.
Keyword
C3H10T1/2 cellsMC3T3-E1 cellsOsteoblast differentiationSulfuretinTGF-beta
ISSN
0300-8177
Publisher
Springer
DOI
http://dx.doi.org/10.1007/s11010-015-2537-5
Type
Article
Appears in Collections:
Division of Biomedical Research > Disease Target Structure Research Center > 1. Journal Articles
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