Thymic stromal lymphopoietin induction is mediated by the major whey proteins α-lactalbumin and β-lactoglobulin through the NF-κB pathway in immune cells

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Title
Thymic stromal lymphopoietin induction is mediated by the major whey proteins α-lactalbumin and β-lactoglobulin through the NF-κB pathway in immune cells
Author(s)
Jae Min Yoo; Y W Park; S Y Yoon; J Y Son; S G Jeong; B Y Park; Jae Wha Kim; M S Nam
Bibliographic Citation
Journal of Agricultural and Food Chemistry, vol. 63, no. 50, pp. 10803-10810
Publication Year
2015
Abstract
α-Lactalbumin and β-lactoglobulin are two major whey proteins that specifically bind immunoglobulin E and are suspected as major allergens causing cow's milk allergy (CMA). Recent studies have shown that thymic stromal lymphopoietin is a critical factor linking at the interface of the body and environment to the T-helper 2 response. However, it is not known whether thymic stromal lymphopoietin expression is changed by α-lactalbumin and β-lactoglobulin in immune cells. Using RT-PCR and ELISA, the present study was conducted to examine if intravenous injection of α-lactalbumin and β-lactoglobulin increased pro-inflammatory cytokines, T-helper 2 cytokines, and thymic stromal lymphopoietin expression in several immune cells, including macrophages, mast cells, and keratinocytes. Results showed that α-lactalbumin and β-lactoglobulin induced thymic stromal lymphopoietin, interleukin-6, and tumor necrosis factor-α expression. It was concluded that the allergenicity of α-lactalbumin and β-lactoglobulin may be attributed to thymic stromal lymphopoietin induction, T-helper 2 cytokines, and pro-inflammatory cytokines.
Keyword
pro-inflammatory cytokineT-helper 2 cytokinethymic stromal lymphopoietinα-lactalbuminβ-lactoglobulin
ISSN
0021-8561
Publisher
Amer Chem Soc
DOI
http://dx.doi.org/10.1021/acs.jafc.5b04790
Type
Article
Appears in Collections:
Division of Biomaterials Research > Cell Factory Research Center > 1. Journal Articles
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